【摘要】 目的建立一种稳定的大鼠脑局灶性梗死模型,用于脑梗死后神经干细胞移植的长期观察。方法将48只清洁级Sprague-Daw ley大鼠,随机分为4组,每组12只大鼠。分别进行假手术(第1组)、单纯结扎左侧大脑中动脉(MCA)(第2组)、同时结扎左侧MCA和颈总动脉(第3组)、同时结扎左侧MCA和颈总动脉伴暂时性夹闭对侧颈总动脉(第4组)的方法制备大鼠脑梗死模型;结扎MCA采取经颞骨局部钻孔开颅,直接结扎MCA终段。通过对各组大鼠术后的神经功能评分、墨汁灌注、氯化三苯四唑氮(TTC)染色、MR成像结果对各组模型进行评价。结果4组大鼠神经功能评分经方差分析,P<0.01,其中第4组与前3组两两比较,差异均有显著性。第1、2组模型墨汁灌注、TTC染色及4周后MR I检查,均未见明显缺血性梗死灶;第3组结果显示梗死灶不稳定、体积较小[(18±10)mm3];第4组模型墨汁灌注、TTC染色观察梗死灶范围局限于大脑皮质,4周后进行MR I测量,大鼠梗死灶明显,体积相对稳定[(85±7)mm3]。第3、4组大鼠梗死体积差异有显著性(P<0.01)。结论通过结扎大鼠同侧MCA和颈总动脉伴暂时性夹闭对侧颈总动脉制作的大鼠脑梗死模型,对大鼠创伤小,梗死灶的位置和体积恒定,可以为脑梗死后神经干细胞移植的研究,提供一种理想的大鼠模型。更多还原

【Abstract】 Objective To develop a stable modal of focal cerebral infarction in rats for study of neural stem cells transplantation. Methods Forty-eight rats were assigned into four groups randomly(twelve rats in each group).Different operations were performed in each group: sham operation((group 1)),ligation of the left middle cerebral artery(MCA)(group 2),ligation of the left MCA and the ipsilateral common carotid arteries(CCA)(group 3),ligation of the left MCA and the ipsilateral CCA followed by the temporary clip occlusion of the contralateral CCA for 1.5 hours(group 4).The models was evaluated by neurological deficits,ink perfusion,TTC staining and MRI.(Results) All rats were in good condition after the operation without mortality for 4 weeks.The neurological deficits showed significant difference by analysis of variance(F =19.161,P < 0.01).No cerebral necrotic lesions showed in group 1 and group 2 by ink perfusion,TTC staining and MR imaging.Small and unstable necrosis((18 ± 10)mm~3) was found in group 3.In group 4,the necrotic area was confined in the cortex,confirmed by ink perfusion and TTC staining.The areas of infarction were(85 ± 7)mm~3 by MRI 4 weeks later which was different from that of group 3(t=15.545,P < 0.01). Conclusion Ligation of the MCA and the ipsilateral CCA followed by the temporary clip occlusion of the contralateral CCA may create a stable model of focal cerebral infarction.Because of its homogeneity of infarction volume and site,and low mortality,it could be used for the study on transplantation of neural stem cells.更多还原