【摘要】 翻译后蛋白质的修饰是目前蛋白质组学研究中的一个重要课题,蛋白质磷酸化是最常见、最重要的一种蛋白质翻译后修饰方式,蛋白质磷酸化和去磷酸化几乎调节着生命活动的整个过程。近年来蛋白质组学技术的发展和应用为磷酸化蛋白质的定性、定量和功能研究提供了必要的技术,使大规模和系统性进行磷酸化蛋白质研究成为可能。本文综述了检测和鉴定磷酸化蛋白质的蛋白质组学方法。更多还原

【Abstract】 It is an important topic to study the post-translational modifications.The protein phosphorylation is one of the commonest and important mode of post-translational modifications in protein.The protein phosphorylation and dephosphorylation almost regulate the whole process of vital movement.In recent years,the development and application of technologies in proteomics offer necessary technologies for the characteristic、quantitative and functional study of phosphorylated proteins.It is capable to study phosphorylated proteins widly and systematically.The detection and identification approaches of protein phosphorylation in proteome are summarized.更多还原