【摘要】 目的:探索强启动子T7在可溶性抗体Fab表达中的作用。方法:利用DNA重组的方法将Fd段及K链基因转移到质粒pT7中,置于T7噬菌体启动子的下游,通过蛋白质印迹法证明pT7-3G9是否有可溶性Fab的表达。结果:pT7-3G9有可溶性Fab的表达;pT7-3G9表达的可溶性Fab比p3G9-L高。结论:强启动子系统可作为可溶性抗体Fab的表达载体,T7启动子在可溶性抗体Fab表达中作用比Lac启动子强。更多还原

【Abstract】 Objective:In order to study the function of T7 promotor in expression of Fab.Methods: The Fd and K chain gene was transferred to expression vector pT7 under the control of T7 promotor.We should prove if soluable Fab expressed in pT7-3G9 by Western blotting.Results:pT7-3G9 can be used to express soluable Fab fragment.The quantity of expression is higher in pT7-3G9 than in p3G9-L.Couclusion:Strong promotor system can be used to express soluable antibody Fab.The role of T7 promotor in expression of soluble anti-Fab antibody is stronger than Lac promotor.更多还原