【摘要】 目的:检测急性白血病(acute leukem ia,AL)病人谷胱甘肽硫转移酶-π(glutath ione-S-transferase-π,GST-π)、多药耐药相关蛋白1(mu lti-drug resistance relevant prote in 1,MRP1)的表达,探讨二者与AL多药耐药(mu lti-drug resistance,MDR)的关系及临床意义,明确二者在AL中的表达有无相关性以及两者表达与AL病人临床特征的关系。方法:采用免疫组织化学S-P法检测80例AL病人及30例正常人外周血单个核细胞GST-π、MRP1表达。运用SPSS10.0统计软件,采用χ2检验、四格表精确概率法、t检验、直线相关分析方法统计结果。结果:GST-π、MRP1在难治组的阳性率均高于初治组和完全缓解组;在初治组的阳性率均高于对照组。GST-π、MRP1在AL难治组中的表达具有高度相关性。GST-π、MRP1表达阳性组完全缓解率低于阴性组。GST-π、MRP1的表达与AL难治组病人年龄、性别、骨髓幼稚细胞比例及外周血白细胞计数无关,且两者在急性淋巴细胞白血病(acute lymphob lastic leukem ia,ALL)组、急性非淋巴细胞白血病(acute non lymphob lasticleukem ia,ANLL)组表达无差别。结论:GST-π和MRP1的高表达与AL临床耐药有关;GST-π和MRP1在难治组中的表达密切相关;GST-π和MRP1表达阳性的AL病人的完全缓解率明显降低,两者可能是影响AL疗效的重要指标;GST-π和MRP1在难治组表达阳性率与年龄、性别、外周血白细胞计数、骨髓幼稚细胞比例可能无关,且在ALL组与ANLL组中差别无统计学意义。更多还原

【Abstract】 Objective:To detect the expression of glutathione-S-transferase-π(GST-π),multi-drug resistance relevant protein1(MRP1) in acute leukemia(AL) and investigate the relationship between their expression and multidrug resistance(MDR),reveal the correlation between the expression of GST-π and MRP1,understanding the relationship between their expression and clinical characters.Methods:S-P immunohistochemical staining method was used to determine GST-π and MRP1 in 80 AL patients and 30 normal subjects.Chi-square test,t-test,F-test and linear correlation analysis were used.Results:The positive percentages of GST-π and MRP1 expression was higher in refractory group than newly diagnosed group and complete remission group.The positive percentage of GST-π and MRP1 were higher in newly diagnosed group than normal subjects group.Statistics showed there was positive correlation between the expression of GST-π and MRP1 in refractory group.The complete remission(CR) rate was lower in positive expression group than negative expression group in GST-π and MRP1.The expression of GST-π and MRP1 had nocorrelation with blood leucocyte quantity and myeloid cell percentage.There was no difference between ALL and ANLL in refractory group.Conclusion:Over expression of GST-π and MRP1 is associated with MDR in AL.There was positive correlation between the expression of GST-π and MRP1 in refractory group.Positive expressions of GST-π and MRP1 were probably important factors that influence treatment effect in AL.The expression of GST-π and MRP1 had nothing with age,sex,blood leucocyte quantity and myeloid cell percentage.There was no difference between ALL and ANLL in refractory group.更多还原