【摘要】 目的:对蒙药格根滴眼剂(简称GNT)进行工艺研究,并初步探讨其稳定性。方法:1.利用正交设计实验,以盐酸小檗碱和藏红花甙-1为考察指标,筛选出最佳工艺条件;2.利用ZTC1+1天然澄清剂调节药液的澄明度;3.通过简单药理实验,筛选药液初步浓度;4.利用pH值和渗透压的检测,筛选辅料。结果:黄柏提取工艺的正交实验L9(34)结果分析,加10倍量蒸馏水提取3次,每次30m in;藏红花提取工艺的正交实验L9(34)结果分析,加14倍量蒸馏水,在60℃条件下,提取2次,每次1.5h浸泡;ZTC1+1天然澄清剂的澄清条件为,先用6%的B组份处理后,再用3%的A组份澄清剂处理;在家兔眼结膜微循环的影响实验表明,与对照组比较,GNT的大、中、小剂量组和可的松眼药水组都有显著性差异(P<0.05),与可的松眼药水组比较,GNT的大剂量组有明显性差异(P<0.05),与小、中剂量组无显著性差异,GNT的小、中、大剂量组之间比较,无显著性差异,根据药理实验,定为中剂量浓度,即黄柏、藏红花浓缩液各1mL,熊胆、牛黄各1g加100mL注射用水;经GNT药液的测定,pH值为6-7之间较稳定,平均渗透压的-0.24,配置等渗溶液需加5%NaC l。结论:对蒙药GNT指定出初步的工艺指标,为今后的研究奠定了基础。更多还原

【Abstract】 Objective:To make technological study of traditional Mongolian drug gegen eye drop（short for GNT in the following）.Methods:1.Select the best technological conditions by the experiment of the orthogonal design,with the index of berberine and crocin-1;2.Make a comprehensive analysis of the conditions of ZTC1+1 natural clarifying agents;3.Select the initial intensity of the liquid by simple pharmacological experiment;4.Adjust pH,check the osmotic pressure,select the supplementary materials.Results:According to the analysis of the result of the abstraction of phellodendron the orthogonal design L₉（3⁴）,put 10 times amount of distilled water into it and abstract for 3 times,each of which 30 minutes.Analyse the result of the abstraction of saffron the orthogonal design L₉（3⁴）,put 14 times amount of distilled water into it and in the condition of 60℃,abstract for twice,each of which soaked for 1.5 hours.The condition of ZTC1+1 natural clarifying agents is,to treat it with 6% of the group B,then to treat it with 3% of the ZTC1+1 natural clarifying agents group A;The experiment which studies the influence of the conjunctiva’s microcirculation of rabbit shows,compared to the contrasted group,there is obvious difference（P<0.05）between the dosage groups of GNT and the groups of cortisone eye drops.In contrast to the groups of cortisone eye drops,the large dosage group of GNT has obvious difference（P<0.05）while the medium and small dosage groups no obvious difference.The comparison of the small,medium and large dosage groups reveals that there is no obvious difference of the three groups.According to the pharmacological experinent,the intensity is fixed as the medium i.e,concentrated liquid of phellodendron and saffron,each of which 1mL;Bear gall powder and Crocus sativus,each of which 1g and add 100mL of water for injection to them;By detecting the liquid of GNT,it is relatively stable when pH is between 6 and 7,when the average osmotic pressure is-0.24.If isotonic solutions is compounded,5% NaCl is needed to be added.Conclusion:To get initial technological index for traditional Mongolian drug GNT and to pave the way for further studies.更多还原