【摘要】 通过对内蒙古呼和浩特地区分离3株奶牛乳腺炎大肠杆菌16S-23S rRNA间隔区的PCR检测、基因测序和序列比较分析,结果表明:分离菌株12C与参考菌株的同源率为99.9%,DG-25A和DG-23A与参考菌株的同源率为98.9%,3株分离菌株的同源性高。应用PCR检测奶牛乳腺炎大肠杆菌的方法简便、快速、特异,该方法也可广泛用于乳房炎其他病原菌的检测。更多还原

【Abstract】 Three isolates obtained from Huhhot region in Inner Mongolia were investigated by PCR,sequences and analyses with oligonucleotide primers designed according to species-specific parts of the 16S-23S rRNA spacer region of the species.The results showed that the sequence homology was 99.9% between isolate 12C and reference strain and it was 98.9% between strain DG25A,DG23A and reference strain.There was higher homology within three isolates.The PCR amplification of speciesspecific sequences of Escherichia coli in the study offers a rapid,specific and sensitivity method to diagnose the mastitis.更多还原