【摘要】 目的:检查热休克蛋白27(HSP27)和c-k it在良性前列腺增生(BPH)和前列腺癌(PCa)组织中表达的差异,分析其与BPH的发生和PCa间的关系,特别是其与PCa的发生、发展及预后和治疗的关系。方法:用免疫组化方法(SP法)检测40例BPH和40例PCa组织中HSP27和c-k it的表达。结果:BPH组织标本中HSP27的反应表现为腺上皮的强阳性表达,同时间质也呈阳性表达。PCa组织标本中HSP27的表达主要定位于腺上皮细胞胞质,PCa组织中HSP27的表达强度高于BPH组织(P<0.05)。BPH标本中c-k it蛋白主要分布于平滑肌细胞的胞质。PCa标本中c-k it蛋白主要表达于腺上皮。PCa组织中c-k it蛋白的表达强度低于BPH组织(P<0.05)。HSP27和c-k it的表达均与肿瘤分级呈负相关(P<0.05),HSP27与临床分期呈正相关(P<0.05),c-k it表达与临床分期呈负相关(P<0.05)。结论:HSP27和c-k it的表达水平与BPH向PCa的发展、转化过程,以及PCa的分级分期关系密切,HSP27和c-k it在PCa组织中的表达,可以判断其恶性程度,为临床判断预后和制定治疗方案提供了重要参考指标。更多还原

【Abstract】 Objective: To examine the differences in the expression of HSP27 and c-kit between benign prostatic hyperplasia(BPH) and prostatic cancer(PCa) tissues and to analyse the relationship between their expression and BPH and PCa,especially the relationship with the occurrence,development,prognosis and treatment of PCa.Methods: An immunohistochemical staining(SP method) for HSP27 and c-kit was undertaken on 40 BPH and 40 PCa tissues samples.Results: Consistent patterns of cytoplasmic staining for HSP27 were seen in all sections of tissue from BPH.The glandular epithelium stained very strongly positively and the stroma stained positively.The staining for HSP27 in PCa tissues was located in the cytoplasm of glandular epithelia,but the expression of HSP27 in PCa was higher than BPH(P<(0.05)).The staining for c-kit in BPH tissues was located in the cytoplasm of smooth muscle cells,and in PCa tissues was located in epithelial cells.The expression of c-kit in PCa tissues was lower than BPH(P<(0.05)).The expression level of both HSP27 and c-kit were decreased with the development of grade of PCa(P<(0.05));HSP27 was increased with the development of clinical stage of PCa(P<(0.05));c-kit was decreased with the development of clinical stage of PCa(P<(0.05)).Conclusion: The expression level of HSP27 and c-kit was highly correlated with the process of the development from BPH to PCa,and also correlated with tumor grades and stages.the expression of HSP27 and c-kit may be used as an important pathological index and may be helpful for the treatment of PCa.更多还原