【摘要】 目的:初步探讨白蛋白与卵黄两种蛋白联合应用于人类精液冷冻保存的效果,以期为人类精液冷冻提供一种更为有效的冷冻保护液。方法:以卵黄甘油柠檬酸钠作为对照组,其内加不同浓度的白蛋白(1、2、3、4、5 g/L)作为实验组,对人类精液进行冷冻保存,冷冻前后运用计算机辅助精液分析系统(CASA)进行精子运动参数分析;在实验组中选用冷冻复苏后精子运动参数最佳的加1 g/L白蛋白的卵黄甘油柠檬酸钠组及卵黄甘油柠檬酸钠对照组,进行精子存活率、膜功能、线粒体功能、超微结构比较分析。结果:加1 g/L白蛋白的卵黄甘油柠檬酸钠组,其精子活率、活力明显高于对照组及其他实验组(P<0.05);精子存活率、精子头部未着色率明显高于对照组(P<0.05);精子琥珀酸脱氢酶活性明显高于对照组(P<0.01);精子超微结构优于对照组。结论:白蛋白与卵黄两种蛋白联合应用冷冻保存人类精液的效果优于单用卵黄,且白蛋白的作用与其使用浓度有关;白蛋白与卵黄联合使用在人精液冷冻保存中可能具有相加和互补功能。更多还原

【Abstract】 Objective: To provide a more effective cryoprotective medium(CPM),effect of union of albumin and egg yolk on human sperm cryopreservation was studied.Methods: Egg yolk-glycerol-sodium citrate was regarded as CPM of the control group and egg yolk-glycerol-sodium citrate with different concentrations of albumin(1,2,3,4,5 g/L) were regarded as CPMs of experiment groups.Before and after cryopreservation,sperm movement parameters were assessed by using computer-aided sperm analyzer(CASA) system,and then egg yolk-glycerol-sodium citrate group added 1 g/L albumin was selected,whose movement parameters were the best among the experimental groups,and egg yolk-glycerol-sodium citrateto group as the control to compare sperm survival rate,membrane integrity,function of mitochondrion and ultrastruction.Results: Sperm in egg yolk-glycerol-sodium citrate added 1 g/L albumin showed significantly higher motility,viability than those in the control group and other experimental groups(P<(0.05)).Sperm in egg yolk-glycerol-sodium citrate group added 1 g/L albumin had significantly higher survival rate,head unpigmenting rate than those in control group(P<(0.05)).Sperm in egg yolk-glycerol-sodium citrate group added 1 g/L albumin manifested significantly higher succinate dehydrogenase(SDH) activity than that in control group(P<(0.05)) and had better ultrastructure than that in control group.Conclusion: Union of two kinds of albumin and egg yolk has better effects on human sperm cryopreservation than those of solitary use of egg yolk.The action of albumin is related to its concentration,and albumin combining with egg yolk may have plus and complementary effects on human sperm cryopreservation.Natl J Androl,2006,12(2):115-119更多还原