【摘要】 目的:通过蛋白质组学技术筛查少精子症患者精浆中特异性生物标志物。方法:用H4和SAX-2蛋白质芯片结合SELD I-TOF-MS对20例少精子症患者及20例生育男性精浆样本进行检测,比较两者间蛋白质图谱表达差异。结果:在H4蛋白质芯片上,少精子症患者精浆与生育男性精浆蛋白质图谱相比,有1处蛋白质峰明显增高,差异有显著性(P<0.05),其相对分子质量(Mr)为11 507.4,有1处蛋白质峰明显降低,差异有显著性(P<0.05),其Mr为11 022.9;在SAX-2蛋白质芯片上,少精子症患者精浆与生育男性精浆蛋白质图谱相比有1处蛋白质峰明显增高,差异有显著性(P<0.05),其Mr为16 751.6。结论:少精子症患者精浆中特异性蛋白质的发现,对探究由少精子症引起的男性不育机制及其临床筛查皆具重要意义。更多还原

【Abstract】 Objective: To screening specific biomarkers of oligospermic candidates from seminal plasma by proteomics technology. Methods: The protein spectra of 20 seminal plasma from oligospermic patients and healthy male individuals were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) to compare different protein expressions.Results: Compared with normal control group,a specific protein peak of molecular weight(11 022.9) in the protein spectra of oligospermic patients’ semen on H4 protein-chip reduced significantly,but the protein peak of molecular weight(11 507.4) elevated apparently.For SAX-2 protein-chip,a specific protein peak of molecular weight(16 751.6) in the protein spectra of oligospermic patients’ semen elevated significantly.Conclusion: Screening specific proteins in seminal plasma of oligospermic patients is important for understanding the mechanism of oligospermia and screening suspected oligospermic individuals.更多还原