【摘要】 目的:探讨饮酒大鼠睾丸总抗氧化能力(T-AOC)、一氧化氮(NO)含量和一氧化氮合酶(NOS)活性的变化与生殖细胞凋亡的关系。方法:20只成年健康SD雄性大鼠随机均分为对照组和实验组,实验组和对照组分别用50%的乙醇溶液和蒸馏水按10 m l/kg每晚灌胃1次连续26 d(两个生精周期)后,用硝酸还原酶法测定睾丸组织中NO含量;用化学比色法测定其T-AOC和NOS活性;原位缺口末端标记(TUNEL)法检测生殖细胞凋亡指数(AI)的变化。结果:与对照组相比,实验组生殖细胞AI增加(P<0.01);睾丸组织T-AOC极显著下降(P<0.01),而NO含量明显上升(P<0.01)、NOS活性显著增强(P<0.01)。结论:大量饮酒能诱导生殖细胞凋亡增加,NOS活性增强导致NO的过量产生及T-AOC的显著下降是其重要原因。更多还原

【Abstract】 Objective: To investigate the relationship between germ cells apoptosis and alterations of total antioxidant capacity(T-AOC),nitric oxide(NO) level and nitric oxide synthase(NOS) activity in the testes of rats submitted to alcohol drinking.Methods: Twenty healthy male Sprague-Dawley rats(3 months old) were randomly divided into two groups: control group and experimental group.50% alcohol and distilled water were administered intragastrically at a dose of 10 ml/kg body weight to two groups of rats respectively.After twenty-six days,the biochemical parameters(T-AOC,NO level and NOS activity) were measured with spectrophotometric determination.The TdT-mediated dUTP-X nick end labeling(TUNEL) technique was used to detect germ cells apoptotic index(AI).Results: Compared with the control group,AI was significantly higher(P<(0.01)) in the experimental group;T-AOC level reduced obviously(P<(0.01)),but NO level and NOS activity increased predominantly(P<(0.01)).Conclusion: The excessive production of NO caused by the increasing of NOS activity and the decreasing of T-AOC may be the main causes that alcohol overtaking induces germ cells apoptosis.更多还原