Objective: To investigate the expression of growth differentiation factor 5(GDF-5) during limb skeletal development of mice and the effect of GDF-5 on bone marrow mesenchymal stem cells in vitro. Methods: The expression of GDF-5 mRNA and protein in mouse fetal limb buds were detected in embryonic day 11.5-15.5(E11.5-15.5) by RT-PCR and Western blotting respectively. Type Ⅱ collagen protein was examined with immunocytochemistry and the sulfate glycosaminoglycan was measured by Alcian blue. Results: During ea...
【基金】
National Natural Science Foundation of China(No. 30471753)
【更新日期】
2007-01-15
【分类号】
R329.2
【正文快照】
INTRODUCTION Growth Differentiation Factor 5 (GDF5), amember of the bone morphogenetic protein(BMP) family and the transforming growth factor β(TGFβ) superfamily, had been implicated as a keyregulatory factor in the development of the skeletalstructures
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