【摘要】 目的:研究共刺激分子4-1BBL分子在人恶性血液病细胞系的表达及其生物学意义。方法:流式细胞仪检测4-1BBL分子在不同的人恶性血液病细胞系的表达;用可介导4-1BBL逆向信号的鼠抗人4-1BBL单抗(mAb1F1)作用于8个不同的人恶性血液病细胞系,细胞计数法和3H-TdR掺入法评价细胞系的体外生长和增殖。结果:FACS结果显示4-1BBL分子在人恶性血液病细胞系上有不同程度的表达,其中髓性白血病细胞系U937、HL60及B淋巴瘤细胞系Raji、Daudi上呈现较高水平的表达。细胞计数和3H-TdR掺入实验结果表明mAb1F1对髓性白血病细胞系U937和HL60,具有较强的促增殖效应。结论:共刺激分子4-1BBL分子在髓性白血病细胞系和B淋巴瘤细胞系上表达率较高;4-1BBL分子可通过介导逆向信号促进髓性白血病细胞系U937和HL60的生长和增殖。更多还原

【Abstract】 Objective: To study the expression and function of 4-1BBL in the cell lines of human hematological malignancies. Methods: The expression of 4-1BBL was detected by FACS analysis and the effects of 4-1BBL molecule in the cell lines of human hematological malignancies were studied by anti-4-1BBL mAb 1F1. Cell proliferation was determined by cell counts manually and 3H-TdR incorporation. Results: FACS analysis showed that 4-1BBL expressed highly in myeloid leukemia cell line U937, HL60 and B Lymphoma cell lines Raji, Daudi. Cell counts and 3H-TdR incorporation assay revealed that mAb 1F1 could induce proliferation of myeloid leukemia cell line U937 and HL60. Conclusion: 4-1BBL molecule is expressed in the myeloid leukemia highly as well as B lymphoma cell lines. 4-1BBL molecule could mediate reverse signal to promote the proliferation of myelocytic leukemia cell line U937 and HL60.更多还原