【摘要】 目的:评价IFN-γ转基因方法与肿瘤内注射重组IFN-γ对荷瘤小鼠肝转移的抑制作用。方法:手术显露脾脏后,经脾注入1×107/ml浓度的小鼠结肠癌细胞株CT26单细胞悬液0.1ml,建立小鼠结肠癌肝转移模型;小鼠分成4组(每组12只),术后第2日起分别进行不同的干预。结果:与生理盐水和空载质粒对照组比较,转基因治疗组和外源IFN-γ给药组对抑制肝转移结节的效应明显(P<0.05);转基因治疗组和外源IFN-γ给药组荷瘤小鼠平均生存期分别为(39.5±5.6)天和(40.8±4.7)天,明显长于生理盐水组(29.0±3.5)天和空载质粒组(31.1±4.7)天。结论:IFN-γ转基因治疗对荷瘤小鼠的肝转移有抑制效应,转基因治疗组能够与重组IFN-γ每日给药组取得相似的抑瘤效果。更多还原

【Abstract】 Objective: To evaluate the anti-tumor effect of IFN-γ gene trasfection or recombinant IFN-γ intraperitoneal injection in tumor-bearing mice. Methods:To establish liver metastasis mice model, 0.1 ml of CT26 colon cancer cells suspension (1 × 107cell/ml) was injected into spleen subcapsularlly in 48 Balb/C mice. The mice were divided into 4 groups and then treated with different methods. Results: Compared with control groups, tumor regression effect of significantly reduced metastasis liver nodules were observed in tumor-bearing mice treated with hIFN-γ gene transfer or daily injection of recombinant IFN-γ(P < 0.05). The mean survival time of tumor-bearing mice treated with hIFN-γ gene transfer or daily injection of recombinant IFN-γ was (39.5 ± 5.6) d and (40.8 ± 4.7) d respectively, which was significantly higher than those of tumor-bearing mice treated with daily saline injections (29.0 ± 3.5d) and injected with the empty plasmid (31.1 ± 4.7) d. Conclusion: The inhibition of liver metastasis and enhanced tumor regression effect is confirmed in tumor-beating mice treated with IFN-γ gene transfer. The therapeutic effect of novel therapy of IFN-γ gene transfer is equal to that of daily intraperitoneal injection of recombinant IFN-γ.更多还原