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bFGF、IGF-Ⅰ对兔关节软骨细胞Ⅱ型胶原表达的影响

Effects of basic fibroblast growth factor and insuline-like growth factor-Ⅰ on the expression of typeⅡ collagen in cultured rabbit articular chondrocytes in vitro

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【作者】 苏新戴心怡张耀娟章宏伟

【Author】 SU Xin, DAI Xin-yi, ZHANG Yao-juan1, ZHANG Hong-wei* (Department of Plastic & Burn Surgery, the First Affiliated Hospital of NJMU, Nanjing 210029; 1Department of Pathogenic Biology, NJMU, Nanjing 210029, China)

【机构】 南京医科大学第一附属医院整形烧伤外科南京医科大学病原生物学系南京医科大学第一附属医院整形烧伤外科 江苏南京210029江苏南京210029

【摘要】 目的:研究碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)、胰岛素样生长因子-Ⅰ(insuline-likegrowthfactor-Ⅰ,IGF-Ⅰ)单独及联合应用对体外培养多次传代的兔关节软骨细胞Ⅱ型胶原表达的影响,探讨传代多次的软骨细胞作为软骨组织工程种子细胞的可行性。方法:第3、5、7代体外培养的兔关节软骨细胞,以常规培养组作为对照组,bFGF(5ng/ml)或和IGF-Ⅰ(100ng/ml)单独刺激及联合刺激作为实验组,免疫组化法测定Ⅱ型胶原的定性表达,并通过图像分析作半定量分析。结果:对照组细胞第5代以后无阳性表达。第3、5、7代细胞在bFGF或IGF-Ⅰ刺激下,Ⅱ型胶原表达强度显著高于对照组(P<0.01);bFGF和IGF-Ⅰ联合作用下,Ⅱ型胶原表达强度均显著高于单独以bFGF或IGF-Ⅰ刺激组(P<0.05)。结论:bFGF、IGF-Ⅰ能促进多次传代软骨细胞Ⅱ型胶原的表达,且联合应用时促进作用更为明显。多次传代软骨细胞在生长因子刺激下,仍有再分化的能力,而有成为软骨组织工程种子细胞的可能。

【Abstract】 Objective: To investigate the effects of single and combined use of basic fibroblast growth factor (bFGF )and insuline-like growth factor-Ⅰ(IGF-Ⅰ)on the expression of typeⅡ collagen in cultured rabbit articular chondrocyte of different passages in vitro. Methods: Cultured chondrocytes of passage 3, 5 and 7 were stimulated by bFGF(5 ng/ml), IGF-Ⅰ(100 ng/ml), bFGF(5 ng/ml)+IGF-Ⅰ(100 ng/ml) respectively. The expression of typeⅡ collagen was tested by immunohistochemistry method qualitatively and analyzed by image analysis semiquantitatively. Results: Regular cultured chondrocytes after passage 5 had no positive expression of typeⅡ collagen. The expression of typeⅡ collagen in the chondrocytes of passage 3, 5 and 7 stimulated by bFGF or IGF-Ⅰwas higher than that of regular cultured group (P < 0.01), and the expression of typeⅡ collagen in bFGF+IGF-Ⅰgroup was significantly higher than that of bFGF or IGF-Ⅰgroup respectively (P < 0.05). Conclusion:bFGF or IGF-Ⅰ promotes typeⅡ collagen expression in multi-passage chondrocytes and the combination use of bFGF and IGF-Ⅰ has synergistic effect. The results suggest that multi-passage chondrocytes stimulated by growth factors are capable to express typeⅡ collagen.

【基金】 南京医科大学创新基金(625MC0306)
  • 【文献出处】 南京医科大学学报(自然科学版) ,Acta Universitatis Medicinalis Nanjing(Natural Science) , 编辑部邮箱 ,2006年05期
  • 【分类号】Q813.11
  • 【被引频次】10
  • 【下载频次】166
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