【摘要】 目标:探讨脂联素在人类皮下和网膜脂肪组织中mRNA表达水平,以及糖皮质激素和PPARγ激动剂对脂联素的调控作用。方法:应用real-time摇RT-PCR方法观察了16名成人腹部皮下和网膜脂肪组织中脂联素mRNA表达水平以及与肥胖的关系。通过体外培养,观察地塞米松(dexamethasone,DEX)和/或PPARγ激动剂(troglitazone,TGZ)对不同部位脂肪组织脂联素的表达和分泌的调控作用。结果:皮下和网膜脂肪组织脂联素mRNA表达丰富但无显著部位差异,网膜脂肪组织脂联素mRNA水平与BMI呈显著负相关(r=0.56,P<0.05)。体外培养24h后,TGZ处理组脂肪组织脂联素基因的表达明显增加(皮下:70%;网膜:102%),且网膜脂肪组织脂联素的分泌明显高于对照组(P<0.05);DEX则下调网膜脂肪组织脂联素mRNA的表达水平36%,TGZ可逆转这种抑制作用。结论:网膜脂肪组织中脂联素mRNA的表达随BMI增加而下降,并受到糖皮质激素及PPARγ激动剂调控。脂联素的部位特异性表达和调控可能是内脏型肥胖连接胰岛素抵抗的重要分子机制。更多还原

【Abstract】 Objective: To investigate the adiponectin mRNA level in human subcutaneous (SC) and omental (OM)adipose tissue and to investigate the effects of glucocorticoids and PPARγagonist on adiponectin in vitro. Methods: SC and OM adipose tissue from 16 adults underwent the measurement of mRNA levels of adiponectin using real-time reverse transcription and polymerase chain reaction (RT-PCR). The influence of dexamethasone(DEX)and troglitazone(TGZ)on adiponectin gene expression and secretion were also studied in vitro. Results: There was no significantly difference in adiponectin gene expression between SC and OM adipose tissue. However, adiponectin mRNA levels in OM tissue were negatively associated with BMI. In vitro, TGZ increased adiponectin gene expression(SC : 77%; OM : 102%), but just increased secretion of adiponectin in OM adipose tissue after incubation for 24 h(P < 0.05). DEX reduced adiponectin expression(by 36%) in OM adipose tissue and its inhibition was reversed by TGZ. Conclusion: Adiponectin mRNA in OM adipose tissue is negatively related to BMI and sharply regulated by glucocorticoids and PPAR agonist. Adiponectin in adipose tissue may play an important role in visceral obesity and impaired insulin sensitivity.更多还原