【摘要】 将来源于Spinacia oleracea的铁氧化还原蛋白(ferredoxin,SoFd)通过吸附的方法固定在碳纳米管(CNT)表面.红外光谱表明SoFd固定在CNT表面后没有变性.循环伏安结果表明,SoFd在CNT表面能进行有效和稳定的直接电子转移反应,伏安曲线上表现出一对良好的、几乎对称的氧化还原峰;式量电位E0′不随扫速(在20—120 mV/s的扫速范围内,其平均值为(-571.7±1.9)mV(vs.SCE,pH 7.0))变化而变化;SoFd直接电子转移的表观速率常数ks为(0.73±0.04)s-1.更多还原

【Abstract】 It is for the first time that Spinacia oleracea ferredoxin（SoFd） immobilized on the surface of the carbon nanotube（CNT） modified glassy carbon（GC） electrode（SoFd-CNT/GC） can undergo effective and stable direct electron transfer reaction.The results of FTIR spectra indicate that the procedures of immobilization SoFd on the surface of CNT does not alter the integrate structure of SoFd or damage SoFd leading to a denatured state and SoFd retained the essential feature of its secondary structure on the surface of CNT.The cyclic voltammetric results indicate that the SoFd-CNT/GC electrode exhibits a pair of well-defined and nearly symmetrical redox peaks in 0.1mol/L phosphate buffer solution（pH 7.0）.Its formal redox potential,E′⁰,is almost independent on the scan rates,the average value is（-571.7±1.9）mV（vs.SCE,pH 7.0） in the phosphate buffer solution in the scan rate range of 20 to 120mV/s.The apparent heterogeneous electron transfer rate constant（k_s） of the direct electron transfer of SoFd is estimated to be（0.73±0.04）s^-1.更多还原