【摘要】 根据黄单胞菌gacA基因的同源性设计简并引物,采用PCR方法从水稻白叶枯病菌(Xanthom onas oryzaepv.oryzae,Xoo)中克隆了gacA同源基因,命名为gacAXoo。序列比较显示,该基因在黄单胞菌中是相对保守的。蛋白质保守结构域搜索表明,GacAXoo属于LuxR家庭的一员,具有与其他GacA蛋白相似的结构。通过同源重组的方法,构建了gacAXoo的插入突变体,为下一步研究gacAXoo的功能奠定了良好的基础。更多还原

【Abstract】 A gacA homologue,designated gacA_Xoo,was cloned from the genomic DNA of Xanthomonas oryzae pv.oryzae(Xoo),a bacterium that causes bacterial blight of rice,with degenerated primers by polymerase amplification reaction(PCR).NCBI blast search indicated that GacA_Xoo had a similar structure to that of other GacA proteins,and had conserved domain of LuxR-like proteins.Sequence comparison showed that gacA_Xoo was conserved in the Xanthomonas genus.A gacA_Xoo disruption mutant was successfully generated by a single cross-over event,selected by Amp resistance on NA medium,and confirmed by PCR and Southern blot.This mutant is a useful tool for further study on the function of gacA_Xoo in X.oryzae pv.oryzae.更多还原