【摘要】 采用实时荧光定量PCR方法研究了中国黄牛背最长肌中capn1mRNA表达与嫩度的关系。结果表明,capn1mRNA表达量与宰后第3天的牛肉嫩度高度相关(r=0.855)。酪蛋白底物酶原分析表明,不同肌肉的μ-calpain潜在酶活性存在很大差异,而m-calpain潜在酶活性差异不大。capn1mRNA的表达可能是影响牛肉嫩度的主要因素。更多还原

【Abstract】 This study was designed to explore the relationship between capn1 and beef tenderness for M.longissimus from Chinese yellow cattle by the method real-time fluorescent quantitative PCR.The results indicated that beef tenderness at day 3 postmortem had a high correlation with the mRNA concentration of capn1(r=0.855).The analysis of casein zymography revealed that the potential proteolytic activities of μ-calpain differed significantly among individual muscles,while those of m-calpain did not differ.Therefore,the expression of capn1 is probably the main contributor to beef tenderness.更多还原