【摘要】 以杉木成熟及未成熟合子胚为外植体,研究了基本培养基、植物激素、合子胚的发育阶段等因素对杉木愈伤组织的诱导及不定芽分化的影响。结果表明:1/2MS+2 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/LKT+30 g/L蔗糖是成熟合子胚诱导愈伤组织的最佳培养基;处于胚胎选择阶段或者组织与器官分化阶段的幼胚,能诱导出松软、晶莹透明的愈伤组织,诱导频率为2%~6%。成熟阶段的幼胚诱导出的愈伤组织的外观形态与成熟合子胚诱导出的愈伤组织相似,愈伤组织的诱导频率随着合子胚发育的成熟而迅速提高;DCR+1.5 mg/L 6-BA+1.5 mg/L KT+20 g/L蔗糖是诱导不定芽发生的最佳培养基。高约1.5 cm的不定芽转入生根培养基,生根率可达100%。更多还原

【Abstract】 Using mature zygotic embryos and immature zygotic embryos as initial explants,the effect of the following factors on induction callus and differentiation adventitious buds: basal medium,plant hormone and development stages of zygotic embryo were studied.Results indicated that the optimal medium for mature zygotic embryos induction callus was(1/2MS)+2mg/L 2,4-D+1.0mg/L 6-BA+1.0mg/L KT+30g/L sucrose.This medium can induce soft,transparent callus from immature zygotic embryos of embryo selection or tissue and organ differentiation stages.The induction frequency was 2%~6%.Immature zygotic embryos of tissue and organ differentiation or embryo mature stages induction callus were similar in state with those of mature zygotic embryo.The callus induction frequency was significantly improved with the maturity of immature zygotic embryos.The optimal medium for differentiation adventitious buds was DCR+1.5mg/L 6-BA+1.5mg/L KT+(20g/L) sucrose.Shoots with 1.5cm high were transfered to rooting medium and rooting frequency reached 100%.更多还原