【摘要】 hs-3菌摇瓶发酵培养过程中,适宜培养时间为16h。研究结果表明,接种量、摇瓶转速、装液量对培养后活菌数及胞外多糖产量都有影响。进一步正交实验得出,摇瓶转速为显著性影响因素,摇瓶培养的适宜工艺组合为:转速60r/min,接种量3%,装液量250mL(500mL三角瓶),通过10L发酵罐小试,hs-3菌活菌数最大为1.12×109cfu/mL,胞外多糖最大产量819mg/L,和摇瓶培养基本一致。更多还原

【Abstract】 In the course of Shake-flask Ferment-cultivating of hs-3,the feasible cultivating were obtained,16 h was feasible.The results indicated that the bacterium yield and exoplysaccharide were effected by rotate speed,inoculation amount ang the quantity of culture medium.The conditions of Ferment-cultivating were obtained by the L933 orthogonal experiment design and analyzing,rotate speed 60 r/min,inoculation 3 %,the quantity of culture medium enclosing 250 mL(500 mL)were the optimum ferment condtions.The bacterium yield and exoplysaccharide were 1.12×109cfu/mL and 819 mg/L in the course of 10 L Fermentation tank-cultivating.更多还原