【摘要】 目的探讨免疫抑制状态下小鼠MD-1表达的影响因素及在皮肤移植中的效应。方法采用小鼠同种皮肤移植模型,以环孢霉素A(CsA)、他克莫司(FK506)、霉酚酸酯(MMF)或雷帕霉素(SRL)为免疫抑制手段。结果CsA和MMF能抑制脾细胞的MD-1表达和增殖反应强度,下调血清IL-2水平和上调IL-10水平,并显著延长皮肤移植物平均存活时间(MST);FK506对IL-10水平无明显影响;而SRL对MD-1表达及IL-2与IL-10水平均无明显影响。MD-1表达水平与脾细胞增殖反应强度和血清IL-2水平呈极显著正相关,和IL-10水平呈极显著负相关。结论CsA、FK506和MMF能抑制小鼠MD-1的表达,其作用与血清IL-2水平下调有关。在免疫抑制状态下,MD-1表达下调与同种皮肤移植物存活时间延长有关,而其表达上调对排斥反应的发生具有一定的指示作用。更多还原

【Abstract】 Objective To investigate factors influencing MD-1 expression and their effects on skin allograft under immunosuppressive condition in mice.Methods C57 to Balb/c skin allograft model was employed in all groups,followed by Cyclosporine(CsA), Tacroli-(mus(FK506)),Mycophenolate Mofetil(MMF),and Sirolimus(SRL) treatment,respectively.Results Compared with saline controls,both CsA and MMF inhibited the MD-1 expression and lymphocyte proliferation,decreased serum level of IL-2 increased serum Level of IL-10,and prolonged survival time of skin allografts.FK506 failed to up-regulate IL-10 level;SRL had no significantly influence on MD-1 expression,IL-2 and IL-10 levels(P < 0.05).The MD-1 expression was positively correlated with lymphocyte proliferation and IL-2 level,but negatively correlated with IL-10 level(P < 0.01).Conclusion CsA,FK506,and MMF can inhibit MD-1 expression.The effects are seemly associated with down-regulation of serum level of IL-2.Under immunosuppressive condition,down-regulation of MD-1 expression is related to prolongation of skin allograft survival.The up-regulation of the MD-1 expression may serve as an indicator for rejection.更多还原