【摘要】 目的研究地塞米松(DEX)诱导小鼠胸腺细胞凋亡进程中线粒体去极化作用特点。方法无菌获取Balb/c小鼠胸腺细胞,设对照组和DEX组;在5 h时点,利用Annexin V-FITC/PI双染流式细胞术检测细胞凋亡;利用DiOC6(3)/PI双染流式细胞术检测细胞线粒体去极化与死亡;利用DiOC6(3)/Annexin V-PE双染流式细胞术检测凋亡过程中的去极化现象。结果在1×10-6mol/L DEX诱导下,小鼠胸腺细胞在5 h凋亡百分率为(36.20±5.11)%,对照组为(4.10±0.98)%,差异显著(P<0.01);DEX组坏死百分率为(4.07±0.24)%,对照组为(1.25±0.25)%,差异显著(P<0.01)。DEX组线粒体去极化增强仍存活的细胞所占百分率为(46.77±6.21)%,显著高于对照组的(12.80±4.55)%(P<0.01)。DEX组线粒体去极化增强且已启动凋亡的细胞为(35.34±4.19)%,显著高于对照组的(7.21±0.61)%(P<0.01)。DEX组线粒体去极化作用增强未凋亡的细胞为(13.68±1.27)%,显著高于对照组的(6.85±0.92)%(P<0.01)。结论DEX诱导小鼠胸腺细胞发生典型细胞凋亡和线粒体去极化增强,在该进程中,线粒体去极化增强发生在细胞膜磷脂酰丝氨酸外翻之前。更多还原

【Abstract】 Objective To study on the dexamethasone（DEX）-induced mitochondrial depolarization and apoptosis process in mouse thymocyte.Mehtods The thymocytes of Balb/c mouse was acquired sterilely,and then divided into control group and DEX(1×10^-6 mol/L) group.At time point of 5 h,the apoptotic and necrotic cells were examined by Annexin VFITC/PI double staining flowcytometry;the depolarization and cell mortality were determined by DiOC6（3）/PI double staining flowcytometry;the depolarization during apoptosis process was tested by DiOC6（3） Annexin V-PE double staining flowcytometry.Results At the time point of 5 h,the apoptotic rate of mouse thymocyte in DEX goup was（36.20±5.11）%,which was significantly higher than that [（4.10±0.98）%] of control group（P < 0.01）;the rate of depolarized cells in DEX group [（46.77±6.21）%] was significantly higher than that [（12.80±4.55）%] of control group（P < 0.01）;the depolarized and apoptotic thymocyte rate in DEX group [（35.34±4.19）%] was significantly higher than that [（7.21±0.61）%] of control group（P < 0.01）.The rates of depolarized but non-apoptotic thymocyte in the two groups had similar trend,which were（13.68±1.27）% and（6.85±0.92）%,respectively（P < 0.01）.Conclusion DEX can induce typical apoptosis and mitochondrial depolarization of thymocytes,and the mitochondrial depolarization occurs before exposure of membrane phosphatidyl serine.更多还原