【摘要】 目的:建立骨髓MSCs体外分离培养体系,并进行骨向分化诱导,以证实其多向分化潜能,为骨髓MSCs进一步的临床应用研究提供实验依据。方法:用密度梯度离心法分离大鼠骨髓MSCs,并对其形态学特征进行观察。用诱导剂对骨髓MSCs向成骨细胞进行诱导分化,并进行形态学观察和免疫细胞化学检测。结果:用密度梯度离心法成功分离获得了高纯度的骨髓MSCs。经骨向诱导后,ALP和矿化结节染色阳性。结论:采用密度梯度离心法成功建立了大鼠骨髓MSCs体外分离和培养体系,并能够向成骨细胞分化。更多还原

【Abstract】 Objective To establish an isolation and culture system of rat bone marrow stem cells and Osteogenic differentiation in vitro in order to verify their pluripotential.Methods Density gradient centrifugation was used to isolate bone marrow stem cells from SD rats.The BMSCs were also undergone osteogenic induction and cytochemical and immunocytochemical staining were performed to verify their multipotential.Results High purity of bone marrow stem cells was successfully obtained.Both ALP activity and mineraliztion node staining were positive in BMSCs after osteogenic differentiation.Conclusion The isolation and culture system of rat bone marrow stem cells was successfully established and the BMSCs sustained their osteogenic differentiation potential in vitro.更多还原