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黄淮麦区小麦品种高分子量谷蛋白亚基组成及遗传多样性分析

Compositions of High Molecular Weight Glutenin Subunits (HMW-GS) and Their Genetic Diversity in Wheat Cultivars from Huang-Huai Wheat Region

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【作者】 王银萍李勇超魏燕燕刘林丽赵惠贤郭蔼光

【Author】 WANG Yin-ping~1,LI Yong-chao~1,WEI Yan-yan~1,LIU Lin-li~2,ZHAO Hui-xian~1,GUO Ai-guang~1(1.College of Life Science,Northwest A & F University,Yangling,Shaanxi 712100,China;2.Biological Technology Laboratory of college of Life Science,Northwest A & F University,Yangling,Shaanxi 712100,China)

【机构】 西北农林科技大学生命科学学院西北农林科技大学生命学院生物技术实验室西北农林科技大学生命科学学院 陕西杨凌712100陕西杨凌712100

【摘要】 为给小麦育种和面粉的深加工提供参考依据,利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法对黄淮五省区50份主推小麦品种的高分子量谷蛋白亚基(HMW-GS)组成进行了研究,并按照Payne等的评分方法计算其Glu-1位点的品质得分。结果表明,黄淮麦区小麦品种中HMW-GS变异较为丰富,在参试材料中共检测到12种不同的亚基类型和17种HMW-GS组合类型,品质平均得分在5.5~7.5分之间。根据HMW-GS组成,可将其聚为3大类,第一大类包括7个品种,其Glu-1品质评分最高,为8~10分;第三大类有4个品种,该类品种品质评分很低,为5分;其它小麦品种被聚于第二大类,其品质评分变化幅度较大,为4~8分。

【Abstract】 The HMW-GS compositions of wheat cultivars from Huang-Huai region were analyzed by SDS-PAGE,the quality scores of each cultivar at Glu-1 were calculated according to the method described by Payne et al. The results showed that the variations of HMW-GS among wheat cultivars in this region were abundance.There were 12 kinds of allelic variations the tested cultivars and 17 combinations of HMW-GS were found.The quality scores of Glu-1 of the tested cultivars in this study were between 5.5 and 7.5.The tested cultivars were classified into 3 groups based on their HMW-GS compositions.The group I includes 7 cultivars and the range of their quality scores was 8~10.The Group III includes 4 cultivars and their quality score was 5.The other cultivars were classified into the group II and the range of their quality scores was 4~8.These results can provide very useful information for rational utilization of these wheat cultivars in wheat improvement.

【关键词】 小麦高分子量谷蛋白亚基遗传多样性
【Key words】 WheatHMW-GSInheritance diversityQuality
【基金】 国家转基因研究与产业化开发专项课题(JY03-A-11-01)
  • 【文献出处】 麦类作物学报 ,Journal of Triticeae Crops , 编辑部邮箱 ,2006年05期
  • 【分类号】S512.1
  • 【被引频次】29
  • 【下载频次】250
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