【摘要】 为了解活性氮积累与小麦白粉病抗性的关系,采用DAB染色法对小麦与不同白粉病菌的互作中活性氧的积累进行了研究。结果表明,小麦对来自白粉病菌附着胞、初生芽管以及次生菌丝的侵染均能够产生活性氧的积累。在感病的小麦品种与白粉病菌的亲和性组合中,活性氧的积累相对较少;在附着胞的侵染位点上活性氧的积累较多;而在受到再次侵染时,表皮细胞侵染位点上产生的活性氧几乎检测不到。在不亲和组合,特别是非寄主组合(小麦/大麦白粉病菌)中,活性氧的积累强度相对较大。同时,不管在什么组合中,气孔周围的短型表皮细胞的活性氧积累往往比长型表皮细胞多,尽管短型的表皮细胞往往表现为更感病。说明活性氧是植物抗病反应的一个指标,与细胞防卫反应的强度有关,但尚不是细胞抗病性唯一的、决定性的因素。更多还原

【Abstract】 Active oxygen accumulation in wheat and powdery mildew fungi infection was examined with DAB reaction.The results showed that active oxygen was detectable on all the infected sites by the fungal primary germ tubes, appressoria,and the infection lobes of growing secondary hyphae.In the compatible interaction,wheat epidermal cells produced relatively less active oxygen;The active oxygen was relatively high on the cells infected by appresorium firstly,and there was no enough active oxygen accumulated to be detected white a haustorium-containing cell was infected again by infection lobes of the hyphae.In the imcompatible interaction and the nonhost interaction between wheat and barley powdery mildew pathogen,much more active oxygen was examined,especially in the nonhost resistant interaction.It was found that,however,more active oxygen was accumulated in short epidermal cells than that in the long ones,even the long cells were more resistant than the short ones were.These suggested that active oxygen just is one of the symbols of plant defense response in wheat against powdery mildew,but it is not the decisive and the exclusive factor of resistance.更多还原