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川麦107慢条锈性遗传分析和分子标记研究
Genetics and Molecular Marker Analysis of Slow-Rusting Resistance to Puccinian striiformis f. sp. tritici in Wheat Variety Chuanmai 107
【摘要】 通过配制杂交组合川麦107×川育12和川麦107×台长29,研究了小麦品种川麦107的慢条锈性遗传特性和分子标记,以期为该品种慢条锈性在新品种选育中的应用提供依据。两个组合F1代植株均表现感病,F2代感病、慢条锈植株呈15∶1分离,表明川麦107慢条锈性受2对隐性基因控制。用已知与Yr5、Yr15、Yr24和Yr26连锁的SSR标记Xgwm501-2B、Xgwm413-1B、Xgwm11-1B和Xgwm18-1B等检测后,没有扩增出相同的条带,表明川麦107可能不含上述4个基因。初步发现了与该品种2个隐性慢条锈基因紧密连锁的SSR标记,即Xgwm46-7B和Xgwm648-3D,其中,Xgwm46-7B位于7B染色体,Xgwm648-3D位于3D染色体,分别与慢条锈性基因相距15.9和20.6cM。
【Abstract】 Genetics of the slow-rusting resistance to yellow rust disease in wheat variety Chuanmai 107 was investigated by the F2 populations of Chuanmai 107×Chuanyu 12 and Chuanmai 107 × Taichang 29”. F1 plants of the two crosses were highly susceptible to the disease whereas segregation ratio of the slow-rust plants to the susceptible plants was 1∶15 in the two F2 populations, which indicated the slow rust resistance in Chuanmai 107 was controled by two recessive genes. No similar bands were amplified by the SSR markers Xgwm501-2B, Xgwm413-1B, Xgwm11-1B, and Xgwm18-1B, which linked with yellow rust resistance genes Yr5, Yr15, Yr24, and Yr26,respectively. But after more extensive screening, 2 SSR markers, Xgwm46-7B on chromosome 7B and Xgwm648-3D Ron chromosome 3D were detected and linked to the 2 recessive slow-rust genes r1 and r2 in Chuanmai 107. The genetic distances between the two markers and two recessive genes slow-rust genes were 15.9 and 20.6 cM ,respectively .the molecular markers of the slow-rust genes would be helpful to slow-rust resistance breeding.
- 【文献出处】 麦类作物学报 ,Journal of Triticeae Crops , 编辑部邮箱 ,2006年03期
- 【分类号】S512.1
- 【被引频次】12
- 【下载频次】154