【摘要】 利用农杆菌介导法将白细胞介素-2基因(il-2)导入番茄中,对影响其转化的因素进行了分析。结果表明:农杆菌菌种(EHA105和C58C1)、外植体类型(子叶和下胚轴)、带有不同筛选标记(Kanr、PPTr、Hygr)的载体质粒几个因素对芽诱导分化及转化均有影响。实验共接种转化2018个子叶和下胚轴外植体,获得了47株抗性再生株,对其进行il-2的PCR扩增检测,有44株呈阳性。PCR-Southern杂交证实PCR结果可靠,显示il-2基因已导入到番茄中。更多还原

【Abstract】 An efficient Agrobacterium-mediated transformation system for transferring Interleukin-2 gene (il-2) into tomato was developed by studying several factors that affect the frequency of shoot differentiation and the efficiency of Agrobacterium mediated transformation of explant tissues. Two Agrobacterium strains (EHA105 and C58C1), two types of explant (cotyledon and hypocotyls)and three vectors with different selectable markers (Kan~r, PPT~r and Hyg~r) were tested for their influences on transformation. Total 2018 cotyledon and hypocotyl explants from three tomato cultivars were transformed by co-cultivation with Agrobacterium, and 47 selective agent-resistant plants were obtained. 44 out of those were il-2 positive by PCR assay. PCR-Southern hybridization of the PCR-tested plants confirmed the PCR assay, indicating that the il-2 gene had been introduced into tomato.更多还原