【摘要】 目的对小鼠阴道黏膜干细胞培养上清中的细胞因子进行初步鉴定。方法收集稳定传代后的小鼠阴道黏膜干细胞培养上清,进行SDS-PAGE电泳和W estern-b lot检测,鉴定培养上清中阴道黏膜干细胞分泌的细胞因子。结果小鼠阴道黏膜干细胞培养上清中含有一种分子量约为84KD的细胞因子,经W estern-b lot方法检测该细胞因子为干细胞生长因子(HGF)。结论小鼠阴道黏膜干细胞可以分泌有活性的HGF。更多还原

【Abstract】 Objective To identify the cytokines in the supernatant of mouse vaginal mucous membrane stem cells.Method To collected the supernatant of mouse vaginal mucous membrane stem cells after VMSCs were subcultured steadly,to detecte cytokines secreted by mouse vaginal mucous menbrance stem cells by SDS-PAGE cataphoresis and Western-bloting methods.Result One kind of cell grath factor with 84kd molecular weight was identified by Western-bloting which was hepatocyte growth factor(HGF).Conclusion VMSCs can secrete active HGF.更多还原