【摘要】 目的探讨环氧化酶-2(COX-2)在新生儿缺氧缺血性脑损伤(HIBD)中的作用及其抑制剂NS398抗凋亡作用。方法新生大鼠HIBD模型为研究对象,半定量RT-PCR检测脑组织COX-2mRNA,免疫组化方法测定COX-2蛋白表达,光镜下观察神经元坏死情况,TUNEL法测定NS398抗凋亡作用。结果HIBD后COX-2表达出现不同程度的增强,在HIBD24h达高峰,同其余各组表达量相比均有显著性差异(P<0.01)。缺氧前、后加入NS398,脑组织神经元凋亡程度有不同程度的降低,与对照组比较有显著性差异(P<0.01),缺氧前给药组神经元凋亡程度低于缺氧后给药组(P<0.01)。结论COX-2在新生儿HIBD形成中发挥重要的作用。NS398具有一定的抗凋亡作用,且早期给予COX-2特异性抑制剂NS398可能更好地发挥其抗凋亡作用。更多还原

【Abstract】 Objective To investigate the effect of COX-2 on the pathogenesis of neonatal HIBD and the effect of NS398 on apoptosis.Methods Neonatal HIBD rat model was established.The brain tissues were collected for microscopic examinantion.The expression of COX-2 mRNA was measured by semi-quantitative RT-PCR,the expression of COX-2 protein was measured by immunohistochemiscal method,the anti-apoptotic effect to sis function of NS398 was estimated by TUNEL.Results The expression of COX-2 was significantly higher than that in control group,peaked at 24?h after hypoxia and ischemia.The neuron apoptosis in 24?h HIBD group was higher significiantly than that in treatment group(NS398),P<0.01.The apoptosis level in the group treated with antagonist NS398 1h before hypoxia was lower than that in the group treated with antagonist NS398 1h after hypoxia,(P<0.01).Conclusion COX-2 plays an important role in the pathogenesis of neonatal HIBD and COX-2 inhibitor has anti-apoptotic effect,which is more effective through early-stage intervention.更多还原