【摘要】 目的探讨哮喘及肺炎患儿血浆白介素-18、-16、-4及IFNγ-水平的相关性及临床意义。方法哮喘患儿22例,肺炎患儿24例(重症组13例,普通组11例)。采用双抗体夹心酶联免疫吸附实验(SELISA)测定其血浆IL-18、IL-16、IL-4、IFNγ-水平。结果(1)哮喘组急性期血浆中IL-16低于非发作期(P<0.05)及对照组(P<0.01),非发作期高于对照组(P<0.01)。非发作期IL-4低于急性期及对照组(P<0.05)。血浆IL-18、IFNγ-无明显改变。急性期IL-18、IL-16、IL-4、IFNγ-水平存在相关性(P<0.01),IL-4与IFNγ-存在相关性(P<0.05)。(2)肺炎组急性期IL-18显著升高,重症组IL-18显著高于普通组(P<0.05)。重症组IL-16显著高于对照组(P<0.05)。各组间IL-4及IFNγ-无统计学差异(P>0.05)。急性期IL-18与IL-16、IL-4、IFNγ-之间存在高度相关性(P<0.01)。IL-16与IFNγ-存在相关性(P<0.05)。结论IL-18、IL-16、IL-4、IFNγ-参与了小儿哮喘和肺炎免疫病理过程。更多还原

【Abstract】 Objective To investigate relationships between plasma levels of IL-18,IL-16,IL-4 and IFN-γ in patients with asthma or pneumonia and clinical significance in children.Methods A total of 22 cases of asthma,24 cases pneumonia (13 severe,11 mild) were recruited into the study,and 20 healthy children as control.IL-18,IL-16,IL-4 and IFN-γ levels were measured by Sandwich ELISA.Results 1.The plasma levels of IL-16 were significantly decreased in patients with asthma at the acute period compared with the convalescent period(P<0.05) and controls(P<0.01);but significantly increased at convalescent period compared with control(P<0.01).The plasma levels of IL-4 were significantly decreased in patients with asthma at the convalescent period compared with the acute(P<0.05) period and control(P<0.05).The plasma levels of IL-18 or IFN-γ had no significant differences between the patient and control groups.At acute period of child patients with asthma,there were correlations with plasma levels of IL-18,IL-16,IL-4 and IFN-γ.2.The plasma levels of IL-18 were significantly elevated in the child patients with pneumonia at acute period compared with the control(P<0.05),and were significantly elevated in the child patients with severe pneumonia at acute period compared with the mild pneumonia(P<0.05) and the control(P<0.01).The plasma levels of IL-16 were significantly elevated in patients with severe pneumonia at acute period compared among the control(P<0.05).The plasma levels of IL-4 or IFN-γ had no significant changes in patients with pneumonia at acute period compared with control.At acute period of pneumonia,there were correlations among the plasma levels of IL-18 and IL-4,IL-16,IFN-γ,and between the plasma levels of IL-16 and IFN-γ.Conclusion The plasma levels of IL-16 were significantly decreased and IL-4 were significantly increased in the child patients with asthma at the acute period.However,at the convalescent period of the asthma,plasma IL-16 was increased.There is an imbalanced Th1 and Th2 cytokine profiles in child asthma.The IL-18,IL-16 levels were significantly elevated in child patients with pneumonia,and the expression of elevated cytokines may reflect severity of disease.更多还原