【摘要】 目的:建立适合影像学评价的稳定可控重复性好的兔局灶性脑缺血再灌注模型。方法:雄性新西兰白兔51只,被随机分为3组,其中A组3只,作为空白对照组;B组8只,为假手术对照组;C组40只,为缺血再灌注组。采用改良颈内动脉线栓法制作模型,用导丝造成大脑中动脉血供阻断(MCAO)2h,缺血再灌注时间点为0h、1h、3h、6h、12h、24h、3d、7d(均n=5),于各时间点对实验动物进行行为评分、磁共振成像及形态学评价。结果:空白对照组、假手术组未见异常信号,缺血再灌注组表现在左侧大脑半球颞顶叶及外侧基底节区域T2WI、DWI出现高信号区,并随缺血再灌注时间延长有逐渐增大趋势,3天后T2WI、DWI高信号范围有所减小。结论:采用该方法和技术指标能够建立稳定、可控、长期存活并适合影像学研究的局灶性脑缺血再灌注模型,为进一步开展缺血再灌注的病理生理变化的研究和MR影像学研究奠定了基础。更多还原

【Abstract】 Objective:To establish a stable,dominated and reduplicated model of focal cerebral ischemia-reperfusion in rabbits,which was suitable for the study of MR imaging especially.Methods:Fifty-one male New Zealand white rabbits were randomly divided into three groups:group A(n=3)underwent no operation,group B(n=8)underwent sham operation,group C(n=40)underwent an operation of ischemic brain injury,in which the occlusion of left middle cerebral artery was induced by guide wire for 2 hours.The time points of reperfusion for group C were 0h,1h,3h,6h,12h,24h,3d,7d(n=5,respectively).At different points,the following examinations were carried out:MR scanning,neurological deficit score and gross anatomy.Results:No abnormal signal was seen on MRI in group A and group B.High signal was seen on DWI and T2WI and high-intensity signal area enlarged with the time and the signal area decreased after 3 day.Conclusions:This method can be adopted to set up a stable,reliable model of focal ischemia-reperfusion.It laid a foundation to further study of pathologic changes of focal ischemia-reperfusion by fundamental medicine and advanced MR technology.更多还原