【摘要】 选用生产上较常用的Ag+、Na+、Ca2+、Cu2+、Mn2+、Pb2+、Zn2+、Fe2+共8种金属盐离子进行试验,研究其在活体条件下对松墨天牛纤维素酶3种组分(Cx酶、C1酶和β-葡萄糖苷酶)活性的影响。结果表明:处理24 h后,Fe2+对纤维素酶有明显的激活作用,离子浓度为1、2 mmol.L-1时Cx酶分别增加4.35%和9.76%,而其余7种离子则抑制纤维素酶的活性,尤其Ag+、Cu2+和Pb2+抑制纤维素酶活性作用效果显著,低浓度时Cx酶分别下降35.20%、36.84%和32.49%;但处理7 d后,参试的8种金属离子对该纤维素酶系表现为一致的抑制作用。随着离子浓度(0.1~50 mmol.L-1)的升高,金属离子的酶抑制效应加剧,Cu2+对Cx酶的抑制作用相应为30.56%~92.55%(短期效应)和25.32%~95.78%(长期效应)。虽然Ag+、Cu2+和Pb2+均有较好的抑制效果,但Cu2+的不可替代的优越性使其在农业生产上具有很好的应用前景。更多还原

【Abstract】 Eight common ions in practice,such as Ag⁺,Na⁺,Ca²⁺,Cu²⁺,Mn²⁺,Pb²⁺,Zn²⁺and Fe²⁺ were selected for experiment.The short-term and long-term effects of ions on the Monochamus alternatus cellulase activity in vivo was studied.The result showed that activity reduced differently when concentration of ions were added except Fe²⁺ in experiment conditions.Among the inhibiting ions, the effect of Ag⁺,Cu²⁺ and Pb²⁺ were evident extremely.Because of heavy metal and high price,Ag⁺ and Pb²⁺ were limited in practice,and not suitable for cellulase inhibitor.None but Cu²⁺and Mn²⁺ can be applied in practice,especially Cu²⁺ have excellent applications potential更多还原