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小蔓长春花离体快繁技术
Study on in Vitro Propagation Techniques of Vinca minor
【摘要】 以小蔓长春花嫩枝的茎段和茎尖为外植体,经90天的增殖培养试验表明,最适宜的增殖培养基为MS+6-BA 0.8~1.2 mg.L-1+IBA 0.2~0.4 mg.L-1,诱导生根的最佳培养基为1/2MS+IBA0.1~0.5 mg.L-1,生根率为96.3%~100%。瓶外生根试验结果表明,用1 000~1 200 mg.L-1IBA蘸根,生根率达81%。
【Abstract】 Tissue culture was made with the stem-segments and shoot tips of Vinca minor as explants.By the multiplication culture of 90 days,the result showed that the optimum medium was MS + 6-BA 0.8~1.2 mg·L-1+ IBA 0.2 ~ 0.4 mg·L-1.The best rooting medium was 1/2 MS+ IBA 0.1~0.5 mg·L-1.The rooting rate was 96.3%~100%.The rooting rate of Vinca minor tissue cultured shoots by treating with 1?000~1?200 mg·L-1 IBA ex virto was 81%.
【关键词】 小蔓长春花;
离体快繁;
增殖和生长;
生根诱导;
瓶外生根;
【Key words】 Vinca minor; In vitro propagation; Multiplication and growth; Rooting of in virto and ex virto;
【Key words】 Vinca minor; In vitro propagation; Multiplication and growth; Rooting of in virto and ex virto;
- 【文献出处】 林业科技 ,Forestry Science & Technology , 编辑部邮箱 ,2006年04期
- 【分类号】S685.99
- 【被引频次】5
- 【下载频次】129