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促进马扎德甜樱桃砧木组培苗生根的研究

Techniques for encouraging rooting of tissue-cultured shoots in sweet cherry on rootstocks ’Mazzard’

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【作者】 范志强李江李成刚谭志坤孙仲序

【Author】 FANG Zhiqiang:Shandong Forestry Bureau,Jinan,250014LI Jiang,LI Chenggang,TAN Zhikun,SUN Zhongxu:Shandong Agricultural University,Taian

【机构】 山东省林业局山东农业大学山东农业大学 济南250014泰安

【摘要】 以甜樱桃砧木马扎德(M azzard)的组培苗为试材,研究促进其生根的方法。结果表明,最佳生根培养基为1/2 MS+0.3mg/L IBA。将分化组培苗转入该培养基,移至温室中黑暗处理10天后,在1500~4000 lx自然光下培养,生根率可达93.5%。对瓶内未生根的幼苗,用1000mg/L IBA浸泡60秒后扦插到温室育苗盘中,1个月后生根率可达80%以上。

【Abstract】 Trials with wild ’Mazzard’ sweet cherry tissue-cultured shoots manifested that the optimal medium for rooting was 1/2MS+0.3mg/L IBA.The rooting rate of 93.5% was obtained by the following practice:the differentiated tissue-cultured shoots were transplanted onto the above-mentioned medium and dark-cultured in a greenhouse for ten days,then subjected to 1500-4000lx of natural light.There was got an additional rooting rate over 80% when the unrooted shoots were immersed in 1000mg/L IBA solution for 60s,then transplanted to nursery trays to be cultured in a greenhouse after one month.

  • 【分类号】S662.5
  • 【被引频次】4
  • 【下载频次】127
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