【摘要】 目的:检测窄谱UVB照射治疗银屑病前后患者外周血IFN-γ、IL-2及IL-4、IL-10水平,探讨窄谱UVB达到银屑病改善缓解的可能免疫学机制。方法:对28名静止期银屑病患者行窄谱UVB照射治疗,观察治疗前、治疗后患者外周血γ-IFN、IL-2和IL-4、IL-10水平的变化,并对其水平和皮损严重度作相关分析。结果:银屑病患者外周血IFN-γ、IL-2水平较健康对照者水平升高,分别为(95.17±8.96)pg/mL比(33.72±3.32)pg/mL和(33.54±2.95)pg/mL比(8.61±1.97)pg/mL(P<0.01)。银屑病患者外周血IL-4、IL-10水平较健康对照者水平降低,分别为(20.70±4.43)pg/mL比(25.42±4.12)pg/mL和(17.86±2.31)pg/mL比(20.92±4.83)pg/mL(P<0.01)。经窄谱UVB照射治疗后,Th1型细胞因子由治疗前的高于健康者的高水平下调,而Th2型细胞因子水平治疗前较正常人水平低,治疗后与治疗前改变无明显差异性。Th1型细胞因子水平和银屑病皮损严重程度呈正相关,Th2型细胞因子水平和银屑病皮损严重程度呈负相关。结论:银屑病是以Th1型细胞因子水平升高Th2型细胞因子水平下降为特点的Th1/Th2免疫反应偏移的免疫性疾病,窄谱UVB可能通过抑制Th1免疫反应相对地逆转银屑病的Th1/Th2反应失衡从而达到临床上银屑病的缓解。更多还原

【Abstract】 Objective:To evaluate serum cytokine profiles by modified enzyme-linked immunosobent assay(ELISA) technique and explore the possiable immune mechanism of NB-UVB(radiation) for psoriasis therapy. Methods:Serum samples from 28 patients with psoriasis before and after NB-UVB radiation were analyzed by colorimetric(EIA) Kits.The disease severity including erythema,induration,scales,and lesion area,were assessed.Results: IFN-gamma and IL-2 were markedly elevated in all psoriasis patients respectively by(95.17±(8.96) pg/mL) versus(33.72±(3.32) pg/mL) and(33.54±(2.95) pg/mL) versus(8.61±(1.97) pg/mL)(P<0.01) in normal controls.Mean IL-4 and IL-10 levels were low detection levels in psoriasis patients compared with controls respectively by(20.70±(4.43) pg/mL) versus(25.42±(4.12) pg/mL) and(17.86±(2.31) pg/mL) versus(20.92±(4.83) pg/mL)((P<0.01)).There are significant increased in the IFN-γ,IL-2 levels but no significant decreases in the IL4,IL-10 levels after NB-UVB radiation.The levels of IFN-γ,IL-2 positively correlated with all indexes of disease severity,the levels of(IL-4),IL-10 were negative.Conclusion:We observed that IFN-gamma and IL-2 cytokines were elevated in psoriasis patients while IL-4,IL-10 were decreased.The polarization of Th2 to Th1 reaction is the one of the pathogenesis of psoriasis.After NB-UVB radiation,the levels of IFN-γ,IL-2 in sera were decreased but the levels of IL-4 and IL-10 in sera were not affected and n-UVB radiation might possibly reverse the polarization to improve the severity of psoriasis.更多还原