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杜仲愈伤组织培养过程中胶含量的动态变化
The Dynamic Change of Gutta-percha Content during Callus Culture of Eucommia ulmoides Oliv.
【摘要】 培养杜仲愈伤组织并测定其含胶量,结果发现,杜仲胶的合成与愈伤组织的生长周期有一定的相关性。在继代培养中,随着继代次数的增加,含胶量呈增加趋势。外植体上愈伤组织一旦形成就有杜仲胶的积累,杜仲胶含量在S5~S8代出现一个高峰值,S8代愈伤组织的含胶量已经达到原植株的94.38%,S9代后又迅速下降。
【Abstract】 This paper reported the dynamic change of gutta-percha content during callus culture of Eucommia ulmoides Oliv.The results showed that there was a relative close relationship existing between the synthesis of gutta-percha and the growth period of calluses.In callus subculture,the gutta content increased with the times of callus subculture.Once callus was formed,the gutta-percha started to accumulate.The gutta-percha content has a peak value from subculture generation 5 to generation 8;it could reach 94.38% of the original plant in subculture generation 8.After generation 9,it declined dramatically.
【Key words】 Eucommia ulmoides Oliv.; Gutta-percha; Callus; Gutta-percha content;
- 【文献出处】 林业科技开发 , 编辑部邮箱 ,2006年05期
- 【分类号】S576
- 【被引频次】4
- 【下载频次】114