【摘要】 用阳离子交换树脂CM-52分离纯化类人胶原蛋白II(HumanCollagen-likeBioproteinII,HCBII)。通过实验,分别确定了静态吸附和柱层析吸附的操作条件,即静态吸附:在pH4.0、NaCl0.15mol/L、进料浓度7g/L,处理量17.26ml/g树脂的条件下进行吸附,吸附时间为60min;柱层析:在pH4.0、NaCl0.15mol/L、进料浓度5g/L、流速5ml/min的条件下进行吸附。然后上柱,在pH4.0、NaCl0.30mol/L下进行脱附。实验表明,采用静态吸附的方式吸附HCBII,然后上柱洗脱,HCBII的吸附量可达到48.6mg/g树脂,回收率83.8%,操作时间短,分辨率高,最终纯化的HCBII达电泳纯,分子量为97kD。更多还原

【Abstract】 A purification method of recombinant human collagen-like Bioprotein II(HCB II) by the CM52 cation exchange chromatography were reported.The batch operation and normal chromatography column method were used in experiments,i.e.,Batch operation of chromatography: HCB II was absorbed under pH 4.0,NaCl(ionic strength) 0.15mol/L,17.26ml(protein volume)/g(resin) and concentration of crude protein liquid 7g/L;Column chromatography: HCB II was absorbed under pH 4.0,NaCl(ionic strength) 0.15mol/L,flow rate 5ml/min and concentration of crude protein liquid 5g/L,the elution of HCB II was pH 4.0,the concentration of NaCl(ionic strength) 0.30mol/L.As a result,the CM52 cation exchange chromatography was of better capacity of adsorption for HCBⅡ and the elution was simple;the total purity process time would be shorten and resolution was high,The recovery of aim protein HCB II with a molecular weight around 97kD was above 83.8% and purity of HCB II could reach electrophoresis grade purity.更多还原