【摘要】 采用自组装单层膜(SAMs)技术在压电石英晶体的金电极表面组装巯基丙酸SAMs,以盐酸1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)作偶联剂,以共价键合方式固定高密度脂蛋白抗体,研制成一种高灵敏的压电免疫传感器用于检测人血清中低含量的高密度脂蛋白。利用压电装置的实时监测功能,考察了巯基丙酸在金电极表面的自组装成膜过程与机制;研究了晶振固定抗体及其反应结合抗原的液相振荡行为和传感特性。传感器采用初始速率法测定高密度脂蛋白的线性浓度范围为1.63~18.8 mg.L-1,检出限为0.82 mg.L-1。更多还原

【Abstract】 It was reported in this paper that a highly sensitive piezo-immunosensor for using in the determination of medium or low contents of HDL in human sera was fabricated.In the fabrication,a monolayer of mercaptopropionic acid（MPA） was assembled on the surface of gold electrode of piezo-electric crystal by the self-assembled monlayer membrane（SAM） technique, during which 1-ethyl-3-（3-dimethylaminopropyl）-carbon diimine and N-hydroxy succinimide were used as coupling reagents and the HDL antibody was bound onto it by covalency.By the initial rate method,the linearity obtained by using this sensor in the determination of HDL was in the range of 1.63-18.8 mg·L^-1 with a detection limit of 0.82 mg·L^-1.Based on the real-time inspection function of the piezoelectric installation,the process and mechanism of the course of SAM of MPA on the surface of the gold electrode was investigated and immobilization of antibody by crystal oscillation,the behavior of liquid phase oscillation of the reaction of binding with antigens and the sensor characteristics were also studied.更多还原