【摘要】 [目的]探讨稀土纳米抗菌材料对红细胞脆性的影响。[方法]依据4种不同的染毒途径,把受试豚鼠分为皮肤接触、经口摄入、肌肉注射和静脉注射4个试验组,前2组所用受试物浓度为50％,后2组所用浓度为10％。于用药第30天和第90天两批处死豚鼠,然后采用红细胞渗透脆性过筛试验,在12支口径相同的试管里顺次滴加NaCI溶液和蒸馏水,观察红细胞的溶血情况。[结果]试验发现豚鼠接触该类稀土纳米抗菌材料后,无论用药第30天还是第90天, 除皮肤接触组外,其他各受试组12支试管均呈深红色,表明红细胞完全溶血,豚鼠红细胞的溶血脆性与对照组相比显著增加。[结论] 红细胞脆性的增加表明在稀土纳米抗菌材料的作用下,红细胞的生物膜结构发生了变化,其抵抗低渗盐水的能力降低,提示过量使用稀土纳米材料会对机体产生不良的影响。更多还原

【Abstract】 [Objective] To study the effect of rare earth anti-bacterial nano materials on erythrocyte fragility. [ Methods ] The guinea pigs were divided into 4 groups, including skin exposure group, oral feeding group, intramuscular injection group and intra veinous injection group. For the former two groups, the concentration of solution was 50%, and 10% for the latter two groups. The guinea pigs tested were killed at 30th and 90th day respectively. The erythrocyte fragility screening test was applied, during testing the solution of NaCl and distilled water was added dropwise, and the hemolytic status was observed. [ Results ] It vas found that except the skin exposure group, the test tubes of all other tested groups appeared deep red, no matter for the tested guinea pigs were killed at 30th day or at 90th day, so the tested rare earth anti-bacterial nano materials caused the obvious increase in erythrocyte fragility excluding in the skin exposure group, compared to the control group. [ Conclusion ] The increase in erythrocyte fragility indicates that upon the action of rare earth anti-bacterial nano materials, the membrane structure of erythrocytes changed and its resistance to lower osmotic NaCl solution decreases. This suggestes that excessive exposure to this material would produce adverse effects to organism.更多还原