【摘要】 目的:检测胃癌、肠化生组织中TGF-βRⅡ(RⅡ)和Bax基因突变与微卫星不稳定性(MSI)发生情况,了解其相关性,进一步探讨MSI在胃癌发生发展中可能的作用机制。方法:酚与氯仿法抽提胃镜下36例胃癌组织标本基因组DNA,二甲苯与蛋白酶K法提取51例肠化生组织石蜡标本基因组DNA,银染PCR-SSCP方法同时检测目的位点。结果:胃癌中RⅡ和Bax基因发生突变分别有7例和6例,MSI发生率达58.3%,两个基因突变均见于MSI阳性的胃癌,尤其是MSI-H型胃癌,而肠化生组织MSI发生率为17.6%,未见RⅡ和Bax基因发生突变。结论:MSI在肠化生-胃癌途径中发挥一定的作用,它可通过引起靶基因突变,特别是部分抑癌基因突变(如RⅡ和Bax基因),促进部分胃癌的发生发展。更多还原

【Abstract】 Objective:By investigating mutations of TGF-β receptor type Ⅱ gene(RⅡ),Bax gene,and the changeable pattern of microsatellite instability(MSI) in gastric cancer(GC)and intestinal metaplasia(IM),the potential mechanism of MSI in the development of GC was explored .Methods:Genomic DNA from thirty-six GC specimen was obtained by endoscopy and fifty-one IM sections cut from paraffin-embedded materials was respectively extracted by phenol-chlorform and xylene-proteinase K.PCR-SSCP(single stand conformation polymorphism)was used to analyze gene mutations and MSI.Results:The mutations of RⅡgene and Bax gene were found in 7and 6 cases of GC,respectively.MSI was identified in 58.3%(21/36)out of GC and 17.6%(9/15)out of IM.Among MSI positive tumors,7(19.2%)showed MSI-H pattern.Two gene mutations were found in GCs with MSI ,especially in GCs with MSI-H.No mutation of RⅡgene or Bax gene was found in IMS.Conclusions:MSI might be important in the development of GC,probably by inducing mutations of target genes such as RⅡand Bax.更多还原