【摘要】 目的检测角膜移植术后肿瘤坏死因子-α受体Ⅰ(tumor necrosis factor alpha receptorⅠ,TNFRⅠ)的含量变化并探讨其与角膜移植免疫排斥反应的关系。方法分别以鸡、兔为供受体建立异种异体穿透性角膜移植模型,对术后不同时间的供受体角膜行TNFRⅠ的免疫组织化学染色,并对其结果进行计算机图像分析,同时观察自体移植组(B)、异体移植CsA治疗组(C)及异种异体移植组(D)各项指标的变化。结果术后10天,D组TN-FRⅠ阳性细胞光密度(optical density,OD)值(0.23)及面积值(63.40μm2)高于B组(q=5.10,P<0.01)及C组(q=3.27,P<0.05);D组发生排斥反应时,OD值及面积值增加,而相应时间B组角膜TNFRⅠOD值及面积值减少,C组变化不明显。结论TNFRⅠ参与角膜移植免疫排斥反应,其含量变化与排斥反应的发生密切相关。更多还原

【Abstract】 Objective To determine the change of tumor necrosis factor-alpha receptor I(TNFRⅠ) content after penetrating keratoplasty and study the relation between TNFRⅠ and corneal graft rejection.Methods We used the chicken to rabbit model of penetrating heterograft.Immunohistochemical and image analysis of TNFRⅠ was performed on cornea in different time points of postoperation.The changes of corneal histopathy were also observed with microscope.Results At post-operative day 10,TNFRⅠ-positive cells were detected at donor-recipient rim in every group.There was no significant difference between the B group and the C group, but TNFRⅠ-positive cells of TNFRⅠ of D group were more than other groups.The positive cells optical density and area of TNFRⅠ in D group increased significantly when the rejection happened.In the same time,we could only find little TNFRⅠ-positive cells at donor-recipient rim in C group and B group,optical density and area of TNFRⅠ-positive cells in C group were higher than that in B group.Conclusions Tumor necrosis factor-alpha receptor Ⅰplayed critical role in corneal graft rejection.The change of TNFRⅠ content associated with the rejection.更多还原