【摘要】 目的:探讨心肌梗死后侧支循环形成与心肌细胞凋亡的关系。方法:建立大鼠急性心肌梗死模型。采用放射性微球法测量局部心肌血流量,HE染色计数血管数,TUNEL法检测细胞凋亡。观察结扎后3 d、7 d、14 d和28 d侧支循环与心肌细胞凋亡的一系列演变过程,采用相关分析两者的关系。结果:梗死周边区凋亡心肌细胞数随时间逐渐降低。梗死区和用边区的局部心肌血流量在结扎后3 d最低,以后逐渐增加,与血管计数的增加相平行。梗死周边区的侧支血流量和血管计数与该区域的凋亡心肌细胞数呈负相关(r分别为-0．961和-0．805,P<0．01)。结论:梗死用边区侧支血管的生长可减少该区域心肌细胞凋亡的发生。更多还原

【Abstract】 Objective:To observe the relationship between coronary collateral circulation and cardiomyocyte apoptosis. Methods: My ocardial infarclion models were induced by ligation of left anterior descending coronary artery (LAD) of SD rats. Eight rats were killed on day 3,7,14,28 after MI respectively. Myocyte apoptosis was assessed by TUNEL assay. Myocardial blood flow was measured with radioactive microspheres. Results: The number of apoptotic myocytes in the infarcted zones (IZ) and border zones (BZ) were decreased gradually. The myocardial blood flow in IZ and BZ increased from day 3 to 28 paralleled with counts of capillary and arteries increasing. Cardiomyocyte apoptosis in the BZ negatively correlated with the myocardial blood flow and the number of capillary and arteries (r was -0. 961 and -0. 805 respectively,P<0.01). Conclusion:The decrease of cardiomyocyte apoptosis may be due to the increase of collateral growth and myocardial blood flow in the BZ.更多还原