【摘要】 目的研究血管紧张素转换酶基因插入(I)/缺失(D)多态性在东莞市T2DM患者与健康人中的分布。方法采用限制性片段长度多形态多聚酶链式反应(PCRRFLP)技术对东莞市200例T2DM患者和93例健康者进行ACE基因内含子16插入/缺失(I/D)多态性检测。结果东莞市T2DM患者ACEDD、II、ID基因型分布频率分别为8.5%、46.0%和45.5%,等位基因频率分别为I:68.8%、D:31.2%。健康对照组的频率分别为19.4%、35.5%和45.2%,等位基因频率分别为I:58.1%、D:41.9%。T2DM患者与健康对照组比较,I等位基因频率显著增高,而D等位基因频率显著降低(P<0.05)。结论ACE基因多态性是致T2DM的遗传危险因素之一。更多还原

【Abstract】 Objective To investigate the distribution of polymorphisms of angiotensin 1-converting enzyme (ACE)in healthy controls and type 2 diabetes mellitus(T2DM) in Dongguan city. Methods The polymorphisms of ACE (I/D) gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 200 T2DM patients and 93 controls in Han’s population of Dongguan city. Results The frequency of I allele of ACE gene polymorphisms was higher in T2DM than that in healthy controls(P<0.05), frequency of D allele was lower in T2DM than that in healthy controls(P<0.05). Conclusions Our results suggests that the ACE I/D gene polymorphism is one of inhered risk factors for T2DM development.更多还原