【摘要】 目的:观察脑缺血再灌注损伤中的细胞凋亡与Bcl2家族的关系。方法:参照ZeaLonga线栓法制作急性大鼠大脑中动脉缺血再灌注模型,观察脑缺血再灌注后大鼠缺血脑组织中的凋亡细胞变化情况及Bcl2、Bax蛋白表达情况,同时通过透射电镜观察缺血侧脑组织神经元超微结构变化。结果:同对照组相比缺血再灌注组凋亡神经元细胞增多(TUNEL阳性细胞)(P<0.05);Bax、Bcl2阳性细胞均升高(P<0.01),同时Bcl2/Bax比值降低;电镜观察神经元超微结构出现凋亡早期改变。结论:Bcl2/Bax比值影响脑缺血再灌注损伤后细胞凋亡。更多还原

【Abstract】 Aim:To observe the apoptosis and Bcl-2,Bax expression on cerebral ischemia-reperfusion (IR) injury.Methods:Rats’cerebral IR injury model was established by Zea Longa’s thread method. Tunel staining,immunohistochmeistry method and electronic microscope were used to observe apoptosis on cerebral IR injury in rats.Results:Compared with control group(sham operation group) ,IR rat model group TUNEL positive cell increased (P<0.05),Bcl-2 and Bax immunoreative neurons increased significantly (P<0.01);the ratio of Bcl-2/Bax was decreased (P<0.01);electronic microscope showed the changes of apoptosis at the early phase in these neurons. Conclusion:The ratio of Bcl-2/Bax influences the apoptosis on cerebral IR injury.更多还原