【摘要】 目的观察戊四氮(PTZ)对神经元样PC12细胞内钙离子(Ca2+)浓度和核因子κB(NF-κB)活化的影响,进一步探讨癫的发病机制。方法将传代培养的神经元样PC12细胞随机分为PTZ干预的PTZ组和不经PTZ干预的对照组,运用免疫荧光法检测神经元样PC12细胞内的Ca2+浓度和NF-κB核区与胞浆区的比值(FN/FC)。结果PTZ组PC12细胞NF-κBFN/FC为0.8821±0.0077,明显高于对照组的0.2161±0.0267(P<0.05),提示NF-κB活化;PTZ组PC12细胞内Ca2+浓度的荧光值(213.45±6.44)明显高于对照组的(80.73±1.01)(P<0.05)。结论PTZ能够使神经元样PC12细胞内Ca2+浓度升高和NF-κB激活。NF-κB激活可能是癫疒间形成的机制之一。更多还原

【Abstract】 Objective To investigate the epileptogenesis by observing effects of pentylenetetrazol(PTZ)on intracellular Ca 2+ concentration and the activation of nuclear factor κappa B(NF-κB)in neuron-like PC12 cells.Methods The subcultured neuron-like PC12 cells were divided randomly into PTZ group treated with PTZ and control group treated without PTZ.The changes of the concentration of intracellular Ca 2+and the expression ratio of NF-κB in nucleus and in cytoplasm(FN/FC)were measured by immunofluorescence assay.Results The relative value of FN/FC of NF-κB in PTZ group(0.8821±0.0077)was higher than that in control group(0.2161±0.0267)in neuron-like PC12 cells(P<0.05).The concentration of intracellular Ca 2+ in PTZ group(213.45±6.44)was higher than that in control group(80.73±1.01, P<0.05).Conclusions PTZ can increases the concentration of intracellular Ca 2+ and activate NF-κB in neuron-like PC12 cells.The activation of NF-κB may play an important role in epileptogeneses.更多还原