【摘要】 目的研究坐骨神经慢性挤压伤(chronicconstrictioninjury,CCI)后不同时点大鼠脊髓磷酸化cAMP反应成分结合蛋白(p-CREB)含量的变化。方法32只160~180g雄性SD大鼠中,8只为正常对照,其余24只做成CCI模型。用VonFrey细丝测定大鼠后爪触诱发痛的变化,并于术后7、14和30d处死(n=8),取L4~L6脊髓用以免疫印迹(Westernblot)方法测定p-CREB的表达量。结果CCI大鼠结扎侧在术后7、14d出现明显的机械感觉异常(P<0·01),30d后基本消失。与正常大鼠相比,CCI大鼠p-CREB含量在术后7、14d均出现明显升高(P<0·01),术后30d恢复到正常水平。结论CCI大鼠疼痛模型中脊髓p-CREB含量明显增高,并且随疼痛症状消失恢复到正常水平。提示脊髓p-CREB在慢性神经性疼痛中发病机制中有一定的作用。更多还原

【Abstract】 Objective To detect the content of phosphorylation of cAMP response element-binding protein(p-CREB) in the spinal cord after chronic constriction injury.Methods Thirty-two male SD rats weighing 160-180g were randomly divided into 4 groups with 8 rats each.Eight rats were selected as control group.CCI model was established in 24 rats and the tests of mechanical allodynia were done by Von Frey filaments.CCI rats were killed at post-operative day of 7(group D7),14(group D14) and 30(group D30) respectively.Lumbar 4-6 spinal cords of CCI and control rats were dissected out and contents of p-CREB were measured by Western blot.Results There were significant increases in the contents of p-CREB in animals exhibiting mechanical allodynia as behavioral sign of neuropathic pain(group D7 and D14).In contrast,following the typical disappearance of pain behavior 30 days after CCi,there were no differences in p-CREB expression content between control and sciatic ligation animals.Conclusion CREB activation may contribute to the development of neuropathic pain in rats induced by sciatic nerve ligation. [更多还原