【摘要】 目的:探讨上皮钙粘素(E-cad)外显子13(Exon-13)C/T单核苷酸多态性与膀胱移行细胞癌的生物学行为之间的关系。方法:对膀胱移行细胞癌患者130例(膀胱癌组)和健康人及非肿瘤患者60例(对照组),采用聚合酶链反应和DNA测序方法,检测包含上皮钙粘素基因Exon-13的DNA片段的基因序列,分析该片段中的SNP。选择χ2检验和t检验进行差异显著性分析,Woolf法计算OR值和95%CI。结果:膀胱癌组E-cad基因Exon-13154bp处T等位基因频率高于对照组(P<0.01);膀胱癌组中浸润性膀胱癌T等位基因频率高于表浅膀胱癌(P<0.05);膀胱移行细胞癌病理分化程度分级与T等位基因频率之间呈正相关(P<0.05)。结论:E-cad基因Exon-13154处T等位基因频率与膀胱移行细胞癌的发生及其侵袭能力密切相关。更多还原

【Abstract】 Objective:To investigate whether single nucleotide polymorphisms in Exon 13 of E-cadherin gene are associated with transitional cell carcinoma of urinary bladder (TCCB).Methods:A hospital-based case-control study was performed on 130 patients with TCCB (male 96 and female 34, age form 29 to 84, mean (58.1±14.7) and 60 normal controls (male 31 and female 19, age form 28 to 81, mean (56.1±14.8). Genotypes were determined using DNA sequencing. SNP in E-cadherin DNA fragments of Exon-13 were analyzed. Cignificance of difference was determined by Chi square test and Student’s t-test. Calculation of OR and 95CI was performed by Woolf method.Results:The T allele frequencies at 154 position of E-cadherin gene Exon-13 were significantly higher in case group than in control group (P<0.01). The T allele frequencies in superficial TCCB patients were higher than those in invasive TCCB patients (P<0.05).Conclusions:The T allele frequencies at 154 position of E-cadherin gene Exon-13 were associated with carcinogenesis and invasive capability of TCCB.更多还原