【摘要】 目的:探讨α-生育酚对精索静脉曲张(VC)大鼠睾丸生殖细胞损害的保护作用。方法:采用成年雄性Wistar大鼠10只复制精索静脉曲张疾病模型,4周后开始每日肌注α-生育酚0.5mg/(100g体重),共4周(生育酚组)。同时设手术组及假手术组进行对照。8周后用原位末端脱氧核苷酸转移酶标记法(TUNEL)检测睾丸生殖细胞凋亡指数(AI)。结果:生育酚组动物左侧睾丸重量显著高于手术组(P<0.05),低于假手术组(P<0.05);生育酚组细胞凋亡率[左侧(5.34±1.28)%,右侧(5.87±1.25)%]高于假手术组[左侧(4.72±1.02)%、右侧(4.88±1.28)%](P<0.05),而显著低于手术组[左侧(9.05±2.51)%,右侧(7.22±1.35)%](P<0.05)。结论:α-生育酚能降低精索静脉曲张大鼠生殖细胞的凋亡指数,延缓睾丸细胞损害的病程,对精索静脉曲张导致的睾丸损害具有一定的保护作用。更多还原

【Abstract】 Objective:To study the effects of α-tocopherol on spermategenic cellular apoptosis in experimental varicocele in rats.Methods:Thirty adult male Wistar rats were randomized into 3 groups(n=10): treated group (TG), operation group (OG), and sham operation group (SOG). Rats in treated group were injected intramusculously α-tocopherol ( 0.5 mg/100 g body-weight, one time daily for 4 weeks) after varicocele bei ng induced by partial ligation of the left renal vein for 4 weeks. All the rats were breed in the similar condition for eight weeks.The apoptosis cells were detected by in situ terminal deoxynucleotityl transferase mediated dTUP nick end lableling(TUNEL).Results:The left vena spermatica of TG and OG were found cirsoid markedly comparing with SOG(P< 0.01 ), the weight of left testicle in TG was intervened between the OG and SOG (P< 0.05 ). The changes of histology were extra marked both in TG and OG. The apoptosis index of TG was lower than that of OG(P< 0.05 ), and higher than that of SOG(P< 0.05 ). Conclusions:It shows that α-tocopherol can decrease the apoptosis index in germ cells of varicocle rats, and slow down the proceeding of testicular injured.更多还原