【摘要】 目的建立对二球悬铃木花粉过敏的特应性体质者的HLA-DRB1等位基因的PCR-SSP检测方法,探讨江苏汉族人二球悬铃木花粉变应原特应性体质者与HLA-DRB1等位基因的相关性。方法用酚-氯仿法提取全血DNA,合成8对等位基因特异性引物,用PCR-SSP方法检测20例江苏汉族二球悬铃木花粉过敏体质者和36例健康人HLA-DRB1*0401,*0402,*0403,*0404,*0405,*0406,*0407,*0408等位基因。结果经优化实验条件,建立了HLA-DRB1的8个等位基因的PCR-SSP检测方法,获得了患者组和健康人组上述HLA-DRB1等位基因的分布资料。二球悬铃木花粉变应原特应性体质者DRB1*0405和*0406基因频率高于健康人对照组而DRB1*0402基因频率低于健康人对照组。其他5个等位基因频率二组间无显著性差异。结论HLA-DRB1*0406和*0405可能是对二球悬铃木花粉变应原过敏的I型变态反应特应性体质者的可疑候选易患等位基因,而DRB1*0402基因可能是与之相关的具有抵抗作用的等位基因。更多还原

【Abstract】 Objective To develop a PCR-SSP method for detection of HLA-DRB1 alleles in the patients who were hypersensitive to Platanus Acerifolia pollen allergen,and to probe into the association between the atopic subjects to Platanus Acerifolia pollen allergen and HLA-DRB1 alleles.Methods DNA in whole blood was extracted by phenol-chloroform method.Eight pairs of specific primers for alleles were synthesized,and HLA-DRB1*0401,*0402,*0403,*0404,*0405,*0406,*0407,*0408 alleles in 20 atopic patients and 36 healthy individuals of Jiangsu Province with Han nationality were detected by PCR-SSP(polymerase chain reaction-sequence specific primer).Results By optimizing the experimental conditions PCR-SSP methods for detection of the 8 alleles were established and the distributing data of above-mentioned HLA DRB1 were obtained.The frequency of HLA DRB1*0405 and *0406 in the patients group was higher than that of in healthy controls group,while the frequency of HLA DRB1*0402 in the patients group was lower than that in controls.No significant deference for the other 5 alleles was found between the 2 groups.Conclusion HLA-DRB1*0406和*0405 seems to be the likely suspected candidate alleles responsible for susceptibility to Platanus Acerifolia pollen allergen in the atopic patients,while DRB1*0402 might be contribute to the related resistance to the allergen.更多还原