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匀浆法提取沙棘果中总黄酮的工艺研究
Homogenated Extraction of Total Flavonoids from Fruits of Sea Buckthorn(Hippophae rhamnoides L.)
【摘要】 为了建立一种高效提取沙棘果中总黄酮的方法,对匀浆法提取沙棘果中总黄酮的工艺进行了研究,确定了最佳的工艺参数:提取原料为含水率85%的沙棘果,溶剂为85%的乙醇,匀浆时间10 m in,液固比为5∶1(mL∶g)。将该法与常规的回流提取法进行了比较。结果表明,在优化的条件下,匀浆法对沙棘果总黄酮的提取率为0.76%,与回流提取相当。匀浆提取法所用提取时间短,提取溶剂用量少,该法是一种高效、快速提取沙棘果总黄酮的方法。
【Abstract】 To establish an efficient method of extracting total flavonoids in the fruits of sea buckthorn(Hippophae rhamnoides L.),homogenated extraction was studied in comparing with reflux extraction.The optimum technology parameters are as follows: fruits(containing 85 % water) of sea buckthorn have to be homogenated for 10 min with 85 % ethanol at a ratio of liquid to solid of 5 ∶1(mL ∶g).Under the optimium condition,extraction yield of total flavonoids is 0.76 % by homogenated extraction,which is equivalent to that of reflux extraction.However,homogenated extraction requires less extraction time and solvent,which is an efficient and rapid method for extracting total flavonoids from the fruits of sea buckthorn.
- 【文献出处】 林产化学与工业 ,Chemistry and Industry of Forest Products , 编辑部邮箱 ,2006年02期
- 【分类号】TQ914.1
- 【被引频次】32
- 【下载频次】524